Friday, January 24, 2020

The yellow wallpaper -- essays research papers

Using examples from all of the texts from this specific unit compare and contrast the conflicts that drive these struggles of the main characters. Look for similarities and look for differences within those similarities. Look for differences and look for similarities within those differences.   Ã‚  Ã‚  Ã‚  Ã‚  In the story â€Å"The yellow wall paper† the main character struggles due to her husband oppression and she suffers herself until getting mental ill. She is put by her husband on a nursery home to be taking care of, but her fear, anxiety and necessity of communication and comprehension from her husband and with the outside world doesn’t make her any better â€Å"I sometimes fancy that in my condition if I had less opposition and more society stimulus-but John says that very worst thing I can do to think about my condition and confess it always makes me feel bad† (507). She is stalwartly hoping to be taken out of the nursery but she had never confronted her husband. â€Å"I wish I could get better† (509). â€Å"But I most not think about that† (509).The yellow wallpaper found in the character’s bedroom grabbed her attention since she first saw it. She found a resemblance of her life and what the wallpaper represents. She w ants to be in her own stated of mind again, but her husband is going to take her physician fro nervous disorder if she doesn’t get better â€Å"John says if I don’t pick up faster he shall send me to Weir Mitchell in the fall† (511). She wishes to be cure but her fears to John don’t allow her to have a confrontation with him. She is very afraid of him and as a consequence, she keeps focusing in the wall paper as a way of escaping from that life that she has.   Ã‚  Ã‚  Ã‚  Ã‚  Ã¢â‚¬Å"The Gilded six-bits† is a story of love, infidelity, and pardon. Joe has a modest but cheerful home. Newlywed, he and his wife Missie May have a joyful and unpretentious life; until a polished and stylish stranger enters into their community demolish their marriage. The infidelity and disloyalty is what makes the main character struggles. Missie May have a relationship with an affluent gay that had moved into their neighborhood â€Å"Oh Joe, honey, he said he wuz gointer give me dat gold money and he jes’ kept on after me† (1278). That was her excuse for the infidelity. Even though Joe was wounded, he never left his wife, â€Å"She loved him too much, but she could not u... ...† (2047). Norma tries to do weights and heavy work while Leroy has a disable condition. They both struggle trying to find who they really are and what they want to accomplish in their life. Finally, after this life change, they realize that their marriage is now devastated and tried to repair by going away. The struggles that they went through made one of them fainted and Norma finally noticing that her life has nonsense and that she doesn’t really know who she is, tries to kill herself.   Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  The story â€Å"Painting Churches†, reveals the story of a complex relationship between a family conformed by aged parent and a young daughter, trying to pursue an artist carrier. While the family tries to move out of their old house, Mags, the young daughter, comes in from Boston to help them move out of the house and at the same she wants to do a portrayal of them â€Å"It’s a perfect opportunity. There will be no distractions; you will be completely at my mercy† (2154). When the time goes by in the story, in between wrapping, rhetoric and painting, it begins to survey deeper into the hostility, grudge and misapprehend of the Church family.

Thursday, January 16, 2020

Fourier Transform Infrared Spectroscopy

Introduction The range of Infrared region Is 12800- 10 cm-l. It can be divided into near-infrared region (12800 – 4000 crn-ll mid-infrared region (4000 – 200 crnl ) and far-infrared region (50 † 1000 cm-l). scientists have established various ways to utilize infrared light. Infrared absorption spectroscopy is the method which scientists use to determine the structures of molecules with the molecules' characteristic absorption of infrared radiation. Infrared spectrum is molecular vibrational spectrum.When exposed to Infrared radiation, sample molecules selectively absorb radiation of pecific wavelengths which causes the change of dipole moment of sample molecules. Consequently, the vibrational energy levels of sample molecules transfer from ground state to excited state. The frequency of the absorption peak is determined by the vibrational energy gap. The number of absorption peaks is related to the number of vibrational freedom of the molecule. The intensity of ab sorption peaks is related to the change of dipole moment and the possibility of the transition of energy levels.Therefore, by analyzing the infrared spectrum, one can readily obtain abundant structure information of a molecule. Most molecules are infrared active except for several homonuclear diatomic molecules such as 02, N2 and C12 due to the zero dipole change in the vibration and rotation of these molecules Concept: Fourier transform spectroscopy Is a less Intuitive way to obtain the same Information. Rather than shining a monochromatic beam of light at the sample, this technique shines a beam containing many frequencies of light at once, and measures how much of that beam Is absorbed by the sample.Next, the beam Is modified to contain a different combination of frequencies, giving a second data point. This process is repeated many times. Afterwards, a computer takes all these data and works backwards to Infer what the absorption Is at each wavelength The beam described above is generated by starting with a broadband light source† one containing the full spectrum of wavelengths to be measured. The light shines into a Michelson interferometer†a certain configuration of mirrors, one of which is moved by a motor. As this mirror moves, each wavelength of light in the beam is periodically blocked. ransmitted, blocked, transmitted. by the Interferometer, due to wave interference. Different wavelengths are modulated at different rates, so that at each moment, the beam coming out of the interferometer has a different spectrum. Fourier Transform of Interferogram to Spectrum The interferogram is a function of time and the values outputted by this function of time are said to make up the time domain. The time domain Is Fourier transformed to get a frequency domain, which is deconvoluted to product a spectrum Step 1: The first step is sample preparation. The standard method to prepare solid sample for FTIR spectrometer is to use KBr.About 2 mg of sample an d 200 mg KBr re dried and ground. The particle size should be unified and less than two micrometers. Then, the mixture is squeezed to form transparent pellets which can be measured directly. For liquids with high boiling point or viscous solution, it can be added in between two NaCl pellets. Then the sample is fixed in the cell by skews and measured. For volatile liquid sample, it is dissolved in CS2 or CC14 to form 10% solution. Then the solution is injected into a liquid cell for measurement. Gas sample needs to be measured in a gas cell with two KBr windows on each side. The gas cell should first be vacuumed.Then the sample can be introduced to the gas cell for measurement. Step 2: The second step is getting a background spectrum by collecting an interferogram and its subsequent conversion to frequency data by inverse Fourier transform. We obtain the background spectrum because the solvent in which we place our sample will have traces of dissolved gases as well as solvent molecul es that contribute information that are not our sample. The background spectrum will contain information about the species of gases and solvent molecules, which may then be subtracted away from our sample spectrum in order to gain nformation about Just the sample.Figure 6 shows an example of an FTIR background spectrum. Figure 6. Background IR spectrum The background spectrum also takes into account several other factors related to the instrument performance, which includes information about the source, interferometer, detector, and the contribution of ambient water (note the two irregular groups of lines at about 3600 cm-l and about 1600 cm-l in Figure 6) and carbon dioxide (note the doublet at 2360 cm-l and sharp spike at 667 cm-l in Figure 6) present in the optical bench.Step 3: Next, we collect a single-beam spectrum of he sample, which will contain absorption bands from the sample as well as the background (gaseous or solvent). Step 4: The ratio between the single-beam sample s pectrum and the single beam background spectrum gives the spectrum of the sample (Figure 7). Advantages: Speed: Because all of the frequencies are measured simultaneously, most measurements by FT-IR are made in a matter of seconds rather than several minutes.This is sometimes referred to as the Felgett Advantage. Sensitivity: Sensitivity is dramatically improved with FT-IR for many reasons. The detectors employed are uch more sensitive, the optical throughput is much higher (referred to as the enable the coaddition of several scans in order to reduce the random measurement noise to any desired level (referred to as signal averaging). ? Mechanical Simplicity: The moving mirror in the interferometer is the only continuously moving part in the instrument. Thus, there is very little possibility of mechanical breakdown. Internally Calibrated: These instruments employ a HeNe laser as an internal wavelength calibration standard (referred to as the Connes Advantage). These instruments are s elf-calibratingand never need to be calibrated by the user.

Wednesday, January 8, 2020

Chromosome Function and Mutation

A chromosome is a long, stringy aggregate of genes that carries heredity information and is formed from condensed chromatin. Chromatin is composed of DNA and proteins that are tightly packed together to form  chromatin fibers. Condensed chromatin fibers form chromosomes. Chromosomes are located within the nucleus of our cells. They are paired together (one from the mother and one from the father) and are known as homologous chromosomes. During cell division, chromosomes are replicated and distributed equally among each new daughter cell. Key Takeaways: Chromosomes Chromosomes are composed of DNA and proteins packed tightly to form long chromatin fibers. Chromosomes house genes responsible for the inheritance of traits and guidance of life processes.Chromosome structure consists of a long arm region and a short arm region connected at a central region known as a centromere. The ends of a chromosome are called telomeres.Duplicated or replicated chromosomes have the familiar X-shape and are composed of identical sister chromatids.During cell division, sister chromatids separate and are incorporated into new daughter cells.Chromosomes contain the genetic codes for protein production. Proteins regulate vital cellular processes and provide structural support for cells and tissues.Chromosome mutations result in changes in chromosome structure or changes in cellular chromosome numbers. Mutations most often have harmful consequences. Chromosome Structure A telomere is a region of the DNA sequence at the end of a chromosome. Their function is to protect the ends of the chromosome from degradation. Here they are visible as highlights at the tips of the chromosomes. Credit: Science Picture Co/Subjects/Getty Images A non-duplicated chromosome is single-stranded and consists of a centromere region that connects two arm regions. The short arm region is called the p arm and the long arm region is called the ​q arm. The end region of a chromosome is called a telomere. Telomeres consist of repeating non-coding DNA  sequences that get shorter as a cell divides. Chromosome Duplication Chromosome duplication occurs prior to the division processes of mitosis and meiosis. DNA replication processes allow correct chromosome numbers to be preserved after the original cell divides. A duplicated chromosome is comprised of two identical chromosomes called sister chromatids that are connected at the centromere region. Sister chromatids remain together until the end of the division process where they are separated by spindle fibers and enclosed within separate cells. Once the paired chromatids separate from one another, each is known as a daughter chromosome. Chromosomes and Cell Division Chromosomes are threadlike structures composed of DNA and proteins. During cell division, chromosomes consist of two arms, or chromatids, which are joined by a centromere. Joined chromatids are called sister chromatids. Credit: Adrian T Sumner/The Image Bank/Getty Images One of the most important elements of successful cell division is the correct distribution of chromosomes. In mitosis, this means that chromosomes must be distributed between two daughter cells. In meiosis, chromosomes must be distributed among four daughter cells. The cells spindle apparatus is responsible for moving chromosomes during cell division. This type of cell movement is due to interactions between spindle microtubules and motor proteins, which work together to manipulate and separate chromosomes. It is vitally important that a correct number of chromosomes be preserved in dividing cells. Errors that occur during cell division may result in individuals with unbalanced chromosome numbers. Their cells may have either too many or not enough chromosomes. This type of occurrence is known as aneuploidy and may happen in autosomal chromosomes during mitosis or in sex chromosomes during meiosis. Anomalies in chromosome numbers can result in birth defects, developmental disabilities, and death. Chromosomes and Protein Production DNA is transcribed and translated to produce proteins. Reverse transcription converts RNA to DNA. ttsz/iStock/Getty Images Plus   Protein production is a vital cell process that is dependent upon chromosomes and DNA. Proteins are important molecules that are necessary for almost all cell functions. Chromosomal DNA contains segments called genes that code for proteins. During protein production, the DNA unwinds and its coding segments are transcribed into an RNA transcript. This copy of the DNA message is exported from the nucleus and then translated to form a protein. Ribosomes and another RNA molecule, called transfer RNA, work together to bind to the RNA transcript and convert the coded message into a protein. Chromosome Mutation Genetic Mutation. BlackJack3D/E/Getty Images Chromosome mutations are changes that occur in chromosomes and are typically the result of either errors that happen during meiosis or by exposure to mutagens such as chemicals or radiation. Chromosome breakage and duplications can cause several types of chromosome structural changes that are typically harmful to the individual. These types of mutations result in chromosomes with extra genes, not enough genes, or genes that are in the wrong sequence. Mutations can also produce cells that have abnormal numbers of chromosomes. Abnormal chromosome numbers typically occur as a result of nondisjunction or the failure of homologous chromosomes to separate properly during meiosis.